[소개]

본 논문은 현재까지도 적용되는 in-gel 상태의 시료 단백질에 대한 bottom-up 방식의 시료 전처리와 관련된 protocol을 소개하는 논문으로 다양한 단백질 질량분석에 있어서 도움이 되는 논문이라 소개합니다.

따라서 본 논문은 단백질 서열 분석 및 proteomics 분석을 염두에 두신 분들에게 도움이 되고자 소개를 시켜드립니다.

[초록]

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

논문링크 : npgrj_nprot_468 2856..2860 (upstate.edu)

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<관련 분석항목>

Peptide mapping, Full amino acid sequencing, N-/C-terminal sequencing, Disulfide bond site determination, Free-thiol 분석, Protein/impurity protein ID, PTMs site determination

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