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[HPLC]Tailing factor -
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Tailing factor
The tailing factor is a measure of peak tailing. It is defined as the distance from the front slope of the peak to the back slope divided by twice the distance from the center line of the peak to the front slope, with all measurements made at 5% of the maximum peak height. The tailing factor of a peak will typically be similar to the asymmetry factor for the same peak, but the two values cannot be directly converted.
The tailing factor is a measure of peak tailing. It is defined as the distance from the front slope of the peak to the back slope divided by twice the distance from the center line of the peak to the front slope, with all measurements made at 5% of the maximum peak height. The tailing factor of a peak will typically be similar to the asymmetry factor for the same peak, but the two values cannot be directly converted.
참고로 실제 HPLC 의 분석 크로마토그램 데이터로 부터 간단히 확인하는 방법은 peak integration 이 완료된 데이터로 부터 얻어진 peak 의 retention time 값과 peak 의 start 그리고 end time 의 각각의 retention time 에 대한 편차 값의 비율이 1에 가까울수록 tailing 정도가 낮다는 것을 의미합니다. 이는 곧 컬럼의 수명 및 peak 분리도를 판단하는데 중요한 기준이 됩니다.