Ion pair chromatography helps separate charged analytes using the popular reverse phase chromatography. Ion pair reagents enhance peak shape and retention time when modifying mobile phase ratios or changing stationary phase is not of much help.

 

Ion pair chromatography는 reverse phase chromatography를 사용하여 전하를 띈 분석물질을 분리하는것을 돕는다.

Ion pair reagents는 이동상의 변화 또는 고정상의 변화가 도움이 되지 않을때 머무름시간과 피크 모양을 향상시킨다.

 

 

 

 

 

 

The technique was developed by Dr. Gordon Shill in 1973. Ion paired reagent has an ionic terminal functional group at the end of a non polar chain. The reagent is added to the mobile phase and given sufficient time to equilibrate with the column. The non polar end of the chain is held strongly to the non- polar stationary phase whereas the charged end sticks out into the mobile phase. Ionic spieces of opposite charge in the mobile phase get immobilized by the charge on the reagent, resulting in neutral ion pairing between the sample molecule and the reagent. This helps in selective retention of charge to analytes in reverse phase HPLC.

 

 

Ion exchange chromatography had been in existence for several years but introduction of Ion pair chromatography offered several advantages:

◾Simple preparation of buffers
◾Wide choice of carbon chain lengths for improved retention and separation
◾Significantly reduced separation time
◾Simultaneous separation of both ionized and non-ionized molecules
◾High reproducibility of results
◾Improved peak shapes

 

Ion exchange chromatography의 장점

 

- 간단한 준비

- 머무름과 분리를 증가하기 위한 탄소사슬길이의 폭넓은 선택

- 분리 시간의 감소

- 이온물질과 비이온물질을 동시에 분리 

- 재현성 있는 결과

- 향상된 피크모양

 

 

Analysis of acidic samples is carried out by adding quatrenary ammonium salts such as tetrabutyl ammonium chloride to the mobile phase

 

산성을 띈 분석물질은 tetrabutyl ammonium chloride 같은 4과 암모늄을 이동상에 첨가하여 사용한다

 

Similarly a negatively charged reagent such as an alkyl sulphonic acid can be used to selectively retain positively charged ionic bases

 

마찬가지로 같은 알킬 설 폰산 등의 음전하 시약을 선택적으로 양전하된 이온 성 염기를 유지하는데 사용될 수있다