일반적으로 단백질 시료나 펩타이드 시료의 MALDI-TOF 분석을 원활히 수행하고자 할때 시료내에 포함된 여러 chemical reagent 의 농도에 대한 가이드 라인입니다.
MALDI-TOF Sample Preparation
Sample preparation for MALDI analysis is usually quick and requires little effort. Buffer exchanges are easily obtained using dialysis and Centricon spin tubes. Below is a list for concentration tolerances of buffers components.
|
Sodium Chloride |
50 mM |
|
Phosphate |
10 mM |
|
Tris Base |
50 mM |
|
Urea |
1 M |
|
Guanidine |
1 M |
|
Azide |
0.1% (v/v) |
|
Glycerol |
1.0% (v/v) |
|
PEG 2000 |
0.1% (w/v) |
|
SDS |
0.01% (w/v) |
|
Triton X-100, RTX-100, NP-40 |
0.1% (v/v) |
|
Tween |
0.1% (v/v) |
|
CHAPS |
0.01% (w/v) |
|
n-Octyl-b-glucopyranoside |
1.0% (v/v) |
|
Zwittergent |
0.1% (v/v) |
|
Lauryldimethylamine oxide (LDAO) |
1.0% (w/v) |
|
HEPES |
50 mM |
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