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[MALDI-TOF] Sample 의 direct spotting 을 위한 가이드 라인

일반적으로 단백질 시료나 펩타이드 시료의 MALDI-TOF 분석을 원활히 수행하고자 할때 시료내에 포함된 여러 chemical reagent 농도에 대한 가이드 라인입니다.
 
MALDI-TOF Sample Preparation
 
Sample preparation for MALDI analysis is usually quick and requires little effort. Buffer exchanges are easily obtained using dialysis and Centricon spin tubes. Below is a list for concentration tolerances of buffers components.
 
 
Sodium Chloride
 
 
50 mM
Phosphate
10 mM
Tris Base
50 mM
Urea
1 M
Guanidine
1 M
Azide
0.1% (v/v)
Glycerol
1.0% (v/v)
PEG 2000
0.1% (w/v)
SDS
0.01% (w/v)
Triton X-100, RTX-100, NP-40
0.1% (v/v)
Tween
0.1% (v/v)
CHAPS
0.01% (w/v)
n-Octyl-b-glucopyranoside
1.0% (v/v)
Zwittergent
0.1% (v/v)
Lauryldimethylamine oxide (LDAO)
1.0% (w/v)
HEPES
50 mM
 
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